Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
ILEX LIFE SCIENCES LLC TRYPSINEDTA P10-024100 6X100ML
PAN-Biotech (10x) Trypsin 0.5% / EDTA 0.2% in DPBS, w/o: Ca and Mg (6 x 100 ml). DESCRIPTION: PAN-Biotech (10x) Trypsin 0.5% / EDTA 0.2% in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme plus EDTA in a 10x concentrated PBS solution. Trypsin-EDTA solution is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture. Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. TRYPSIN: 0.50% Trypsin. EDTA: 0.20% (5.3 mM) EDTA. STERILE: Yes. STORAGE TEMP.: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
Bioworld ADA (N-(2-acetamido)iminodiacetic acid) Buffer, 0.2M, pH 7.0, 500 mL
Synonym:ADA-Na,N-(2-Acetamido)iminodiacetic acid monosodium salt,N-(Carbamoylmethyl)iminodiacetic acid monosodium saltA zwitterionic buffer used in biological applications including protein isolation and characterizationWarning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption. Appearance: LiquidPH Value: 7.0
![Alkali Scientific MOPS [3 (N morpholino) propanesulfonic acid], 10 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661636.jpg-250.jpg)
Alkali Scientific MOPS [3 (N morpholino) propanesulfonic acid], 10 Kg
MOPS (3-(N-morpholino)propane-1-sulfonic acid) is a biochemical reagent used for pH buffering in a variety of laboratory applications. This 10kg package of MOPS is ideal for large-scale production of buffer solutions, making it suitable for high-volume research and industrial applications. MOPS buffers are particularly effective in maintaining a stable pH range of 6.5 to 7.9, which is critical for biological assays, enzyme reactions, and protein analysis. It is commonly used in molecular biology, electrophoresis, and chromatography to stabilize proteins, nucleic acids, and enzymes. This bulk packaging ensures a cost-effective solution for labs with high buffer consumption.
Alkali Scientific SSC Buffer, 20X Solution, 1 L
SSC Buffer 20X Solution (1 L) is a ready-to-use, concentrated buffer solution widely used in DNA hybridization experiments. This 20X concentration allows for easy dilution to the desired working concentration in hybridization assays, including Southern and Northern blotting. The high ionic strength provided by SSC Buffer stabilizes nucleic acid interactions, ensuring that probes bind specifically to complementary sequences during hybridization. The 1-liter volume is suitable for routine laboratory use, offering a convenient quantity for hybridization experiments and nucleic acid detection protocols. It is an essential reagent in molecular biology for accurate and reliable results.
Alkali Scientific Murashige & Skoog Medium with MES Buffer, 4.8 Grams of Powder, Makes 1 Liter of Solution
Murashige & Skoog Medium with MES Buffer is a specialized medium designed for plant tissue culture applications that require a stable pH environment. The 1-liter solution, made from 4.8 grams of powder, includes MES buffer, which helps stabilize the pH of the culture medium during growth. This formulation is critical in preventing pH shifts that could negatively affect plant cell growth. The MES buffer is ideal for studies in plant biotechnology, regeneration, and cell culture, where pH stability is essential for proper cellular functions. This medium is commonly used in laboratories focusing on plant tissue culture and genetic research, ensuring consistency in experimental conditions.
Cayman Chemical TrIs Buffer ConcentrAT10X 10mL
A thioredoxin inhibitor inhibits Trx activity in ES-2 cells at 20 UM cytotoxic to ES-2 cells (IC50 1.6 UM) induces ferroptosis in ES-2 cells at 5 UM an effect that can be blocked by DFO Trolox or ferrostatin-1 decreases tumor volume in a 4T1 murine mammary carcinoma model using immunocompetent but not immunodeficient mice at 50 mg/kg twice per week
Seracare Life Sciences Inc 10X TRIS-GLYCINE-SDS BUFFER
Tris-Glycine-SDS (TGS) is a running buffer for sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. pH=8.3. Conductivity controlled.
Crescent Chemical Co Inc TRIS(HYDROXYMETHYL)METHYLGLYCI
Tris(hydroxymethyl)methylglycine analytical grade MP 179 - 187 °C; pKa 20 = 8.15. Buffering substance (1). Non-toxic substitute for barbital (2). Size - 500G Storage Conditions - +15 °C TO +30 °C Catalog Number - 37195.03 CAS 1389-47-5
ATHENA ES HBS w/Phenol Red, 500 ml
Hepes Buffered Saline is available with or without Phenol Red. It is an isotonic saline buffered with Hepes, for use in rinsing cell monolayers prior to cell dissociation using PETTM or washing harvested cells.
Bioworld BES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) Buffer, 0.5M, pH 6.0, 500 mL
Synonym: N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt, BES sodium saltBelongs to the class of Good buffers, as described in the research of Dr. Norman Good et al. (1966), optimized for molecular biology and biochemistry applications.The characteristics of a Good′s buffer include: pKa between 6.0 and 8.0, high solubility, non toxic, limited effect on biochemical reactions, very low absorbance between 240-700 nm, enzymatic and hydrolytic stability, minimal changes due to temperature and concentration, limited effects due to ionic or salt composition of the solution, limited interaction with mineral cations and limited permeability of biological membranes.Technical Information:pKa (25 °C): 7.1 (Free acid)Warning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption.Appearance: LiquidPH Value: 6.0