Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Biotang Inc TRIS-HCL Buffer 1.0M, pH 8.0 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 8.0 (0.2 micron filtered), 500 ml.
Biotang Inc TRIS-HEPES-SDS Running Buffer (10X), 500 ml
TRIS-HEPES-SDS Running Buffer (10X), 500 ml.
Biotang Inc TRIS-HCL Buffer 1.0M, pH 7.4 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 7.4 (0.2 micron filtered), 500 ml.
Biotang Inc TRIS- Tricine -SDS Running Buffer (10X)(Anode Buff), 500 ml
TRIS- Tricine -SDS Running Buffer (10X)(Anode Buff), 500 ml.
Biotang Inc BioAcryl-P (30%) Bis-Acrylamide 29:1, 1000 ml
BioAcryl-P (30%) Bis-Acrylamide 29:1, 1000 ml.
Bioworld TT - SDS Running Buffer (Anode) (10X), 1 L
Product DescriptionTris-Tricine (TT)-SDS running buffer has a 10X concentration and a pH of 8.9. It contains 1M Tris-base, 1M Tricine and 1% SDS.The tricine system requires SDS in sample and running buffers for best results. Tricine-SDS PAGE is commonly used to separate proteins that range in molecular weight between 1 – 100 kDa but is preferred for the resolution of proteins smaller than 30 kDa.
Bioworld ACE TBE w/ 7M Urea, pH 8.6, 100 mL
Product DescriptionA.C.E. TM TBE (Tris-Borate-EDTA) buffer comes with 7M of Urea with a pH level of 8.6. A.C.E. TM buffers deliver consistent and reproducible results because they are manufactured with the highest quality reagents in nuclease free water. Product is shipped at room temperature.
eMolecules 5704-04-1 | Tricine | Oakwood Chemical | MFCD00004277 | 179.172 | C6H13NO5 | 98.000 | OCC(CO)(CO)NCC(O)=O | 1g | 537694332
Tricine | Oakwood Chemical | 5704-04-1 | MFCD00004277 | 179.172 | C6H13NO5 | 98.000 | OCC(CO)(CO)NCC(O)=O | 1g | 537694332
Boca Scientific Inc Coating Buffer, pH 7.4, Based on PBS, 500ml
Coating Buffer is designed for adsorptive immobilization of proteins and antibodies on plastic surfaces, such as microtiter plates, or other protein binding surfaces. Depending on the protein or the antibody, the pH of the Coating Buffer is crucial for efficient immobilization, as the pH-value can have an influence on the steric structure of proteins or antibodies. Coating Buffer is offered with a pH of 7.4, based on PBS, or 9.6, based on carbonate. For the optimization of the coating procedure of a newly developed immunoassay, we strongly recommend testing the two Coating Buffers side by side. Depending on the type of surface and the type of proteins or antibodies, the required incubation time can vary.
Alkali Scientific TRIS CAPS Buffer 10X Solution, 2 x 500 Milliliter Bottles
TRIS CAPS Buffer 10X Solution is designed for use in capillary electrophoresis and other advanced electrophoretic techniques. This formulation, available in two 500mL bottles, ensures convenient preparation and consistent results during capillary separation of charged biomolecules. The 10X solution can be diluted to create the working concentration needed for various electrophoresis applications. TRIS CAPS buffer is particularly useful in separating proteins and nucleic acids in a high-resolution format. This buffer is often used in protein and DNA/RNA electrophoresis protocols, providing stable pH conditions that enhance separation and improve sensitivity during detection.
![Alkali Scientific TBE 10X Solution [TRIS borate EDTA 10X Solution], 1 L](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661163.jpg-250.jpg)
Alkali Scientific TBE 10X Solution [TRIS borate EDTA 10X Solution], 1 L
TBE 10X Solution is a buffered solution commonly used in molecular biology and biochemistry. This 1-liter bottle contains a highly concentrated solution of Tris, borate, and EDTA (ethylene diamine tetraacetic acid), which is used as a running buffer for agarose gel electrophoresis. TBE helps maintain the pH and ionic strength of the electrophoresis buffer system, which is critical for the proper migration of nucleic acids. This 10X concentrated solution can be diluted to 1X for use in standard gel electrophoresis procedures. It is an essential reagent for DNA analysis, particularly in applications involving high-resolution separation of nucleic acid fragments.
Bioworld Bis-Tris Propane, 1 kg
A non-zwitterionic buffer that is useful in the pH 6.3-9.5 rangeSynonym: 1,3-Bis[tris(hydroxymethyl)methylamino]propane Appearance: SolidBoiling Point : 573.3 °CDensity : 1.326 g/cm3IUPAC_Name: 2,2'-(1,3-Propanediyldiimino)Bis[2-(Hydroxymethyl)-1,3-Propanediol]Melting Point: 164-165 °CPH Value: 6.3 - 9.5